| Autor: |
Kassem, Issmat I., Helmy, Yosra A., Kathayat, Dipak, Candelero-Rueda, Rosario A., Kumar, Anand, Deblais, Loic, Huang, Huang-Chi, Sahin, Orhan, Zhang, Qijing, Rajashekara, Gireesh |
| Zdroj: |
Foodborne Pathogens & Disease; August 2017, Vol. 14 Issue: 8 p472-477, 6p |
| Abstrakt: |
AbstractWe investigated the contribution of litter to the occurrence of Campylobacteron three broiler farms, which were known to have low (LO) and high (HI-A and HI-B) Campylobacterprevalence. For this purpose, we collected litter samples (n= 288) during and after two rearing cycles from each farm. We evaluated the occurrence of Campylobacter(using selective enrichment and quantitative real-time polymerase chain reaction [q-PCR] analysis) in the litter samples as well as the litter's pH and moisture content. Ceca from each flock (n= 144) were harvested at slaughter age and used to quantify Campylobactercolony-forming units (CFUs). Campylobacterwas only retrieved from 7 litter samples that were collected from HI-A and HI-B during the growing period, but no Campylobacterwas isolated from LO farms. The q-PCR analysis detected Campylobacterin pooled litter samples from all three farms. However, in litter collected during the same rotation, Campylobacterlevels were significantly higher (p< 0.05) in HI-A and HI-B litter samples in comparison to those in LO. Cecal samples from HI-A and HI-B yielded relatively high numbers of CampylobacterCFUs, which were undetectable in LO samples. Litter's pH and moisture did not affect the overall occurrence of Campylobacterin litter and ceca on any of the farms. Our data suggest that Campylobacterwas generally more abundant in litter that was collected from farms with highly colonized flocks. Therefore, better approaches for assessing the occurrence of Campylobacterin litter might be warranted in order to reduce the dissemination of these pathogens on and off poultry farms. |
| Databáze: |
Supplemental Index |
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