Extracts from Marine Macroalgae and Opuntia ficus-indicaCladodes Enhance Halotolerance and Enzymatic Potential of Diazotrophic Rhizobacteria and Their Impact on Wheat Germination Under Salt Stress

Autor: RAI, Abdelwahab, CHERIF, Ameur, CRUZ, Cristina, NABTI, Elhafid
Zdroj: Pedosphere; April 2018, Vol. 28 Issue: 2 p241-254, 14p
Abstrakt: Soil salinity, which affects more than 6% of the earth's land surface and more than 20% of its irrigated areas, is a major threat to agriculture. Diazotrophic bacteria are among the functional groups of soil microbiota that are threatened by this abiotic stress, as their activity is mostly inhibited by salt stress. Seventy bacterial strains with distinct characteristics were isolated from soils by using N-free Jensen's selective medium. Based on their ability to produce metabolites of agricultural interest, four strains were selected and identified as Flavobacterium johnsoniae, Pseudomonas putida, Achromobacter xylosoxidans,and Azotobacter chroococcum.The selected strains were grown at different NaCl concentrations (0–600 mmol L–1in N-free broth and 0–2 000 mmol L–1in Luria-Bertani medium) in the presence and absence of glycine betaine (GB), aqueous and hydro-alcoholic extracts from marine macroalgae, Ulva lactucaand Enteromorpha intestinalis,and Opuntia ficus-indicacladodes. The selected bacterial strains, GB, and the aforementioned extracts were tested for their ability to promote the germination of wheat (Triticum durum) seeds at 0–300 mmol L–1NaCl. Compared with the results obtained with the synthetic osmoprotectant GB, the extracts from O. ficus-indica, U. lactuca,and E. intestinalissignificantly promoted bacterial growth and seed germination under salt stress.
Databáze: Supplemental Index