| Autor: |
Abd Wahid, Muhamad Azhar, Megat Mohd Noor, Megat Johari, Goto, Masafumi, Sugiura, Norio, Othman, Nor’azizi, Zakaria, Zuriati, Ahmad Mohammed, Thamer, Jusoh, Ahmad, Hara, Hirofumi |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; August 2017, Vol. 81 Issue: 8 p1642-1649, 8p |
| Abstrakt: |
The natural coagulant Moringa oleiferalectin (MoL) as cationic protein is a promising candidate in coagulation process of water treatment plant. Introducing the gene encoding MoL into a host, Pichia pastoris, to secrete soluble recombinant protein is assessed in this study. Initial screening using PCR confirmed the insertion of MoLgene, and SDS-PAGE analysis detected the MoL protein at 8 kDa. Cultured optimization showed the highest MoL protein at 520 mg/L was observed at 28 °C for 144 h of culturing by induction in 1% methanol. Approximately, 0.40 mg/mL of recombinant MoL protein showed 95 ± 2% turbidity removal of 1% kaolin suspension. In 0.1% kaolin suspension, the concentration of MoL at 10 μg/mL exhibits the highest turbidity reduction at 68 ± 1%. Thus, recombinant MoL protein from P. pastorisis an effective coagulant for water treatment. |
| Databáze: |
Supplemental Index |
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