| Autor: |
Roiban, Gheorghe-Doru, Sutton, Peter W., Splain, Rebecca, Morgan, Christopher, Fosberry, Andrew, Honicker, Katherine, Homes, Paul, Boudet, Cyril, Dann, Alison, Guo, Jiasheng, Brown, Kristin K., Ihnken, Leigh Anne F., Fuerst, Douglas |
| Zdroj: |
Organic Process Research & Development; 20240101, Issue: Preprints |
| Abstrakt: |
An epoxide resolution process was rapidly developed that allowed access to multigram scale quantities of (R)-2-butyl-2-ethyloxirane 2at greater than 300 g/L reaction concentration using an easy-to-handle and store lyophilized powder of epoxide hydrolase from Agromyces mediolanus. The enzyme was successfully fermented on a 35 L scale and stability increased by downstream processing. Halohydrin dehalogenases also gave highly enantioselective resolution but were shown to favor hydrolysis of the (R)-2epoxide, whereas epoxide hydrolase from Aspergillus nigerinstead provided (R)-7via an unoptimized, enantioconvergent process. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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