| Autor: |
Elbaz, Elzahara, Moustafa, Mohamed Abdallah Mohamed, Lee, Kyunglee, Mohamed, Wessam Mohamed Ahmed, Nakao, Ryo, Shimozuru, Michito, Sashika, Mariko, Younis, Emad Elsayed Ahmed, El-khodery, Sabry Ahmed, Tsubota, Toshio |
| Zdroj: |
Ticks and Tick-borne Diseases; 20240101, Issue: Preprints |
| Abstrakt: |
Babesiaand Theileriaspecies are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesiaand Theileriaspecies by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87.7% (236/269) and 11.5% (31/269) of the examined PCR products hybridized with the probes which were designed to detect all Babesiaand Theileriaspp., all Theileriaspp., all Babesiaspp., Theileriasp. Thrivae and Babesia divergens-like, respectively. The 18S rRNA gene partial sequences were divided into Theileriasp. Thrivae, T. capreoli, B. divergens-like and an undescribed Babesiaspecies. This study showed the first detection of the undescribed Babesiasp. from Japan. Therefore, more studies are required to understand the ecology of the newly detected tick-borne pathogens in Hokkaido. |
| Databáze: |
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