| Autor: |
Brooks, P J, Kaplitt, M G, Kleopoulos, S P, Funabashi, T, Mobbs, C V, Pfaff, D W |
| Zdroj: |
Journal of Histochemistry and Cytochemistry; December 1993, Vol. 41 Issue: 12 p1761-1766, 6p |
| Abstrakt: |
We describe a procedure for detection of low-abundance cellular RNAs by in situ hybridization histochemistry, using single-stranded DNA probes produced by amplified primer extension labeling with Taq polymerase. We have used this approach to detect a number of high- and low-abundance RNA species and have found it to be a simple and reproducible method of obtaining sensitive probes for in situ hybridization studies. For example, DNA probes generated by amplified primer extension labeling can detect low-abundance heteronuclear RNAs in individual neurons. Since this procedure does not involve recombinant DNA technology or microbiological facilities, it should prove useful to a wide variety of investigators studying the regulation of gene expression at the cellular level. |
| Databáze: |
Supplemental Index |
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