| Autor: |
Ko, YH, Ha, YR, Kim, JW, Kang, SG, Jang, HA, Kang, SH, Park, HS, Cheon, J |
| Zdroj: |
Journal of International Medical Research; June 2011, Vol. 39 Issue: 3 p729-739, 11p |
| Abstrakt: |
This study investigated the mechanism of action of a gonadotropin-releasing hormone (GnRH) agonist, leuprolide, on proliferation of the hormone-refractory prostate cancer cell line DU145, transfected with short hairpin RNA (shRNA), to reduce expression of the GNRHR1gene (which encodes the GnRH type 1 receptor). DU145 cell proliferation in the presence of leuprolide (10−9and 10−7M) or control medium was measured before and after GnRHR1 knockdown. Reverse transcription—polymerase chain reaction and Western blot analysis were used to measure the degree of GNRHR1silencing. DU145 cells treated with leuprolide (10−9and 10−7M) showed significant growth inhibition compared with control-treated DU145 cells. Transfection with GNRHR1–shRNA significantly decreased GNRHR1expression at 48 h. DU145 cells transfected with silencing GNRHR1–shRNA showed normal growth patterns; however, there was no significant inhibition of proliferation of DU145 cells transfected with GNRHR1–shRNA compared with cells transfected with control—shRNA in response to leuprolide. These data demonstrated that the antiproliferative effect of leuprolide was mediated by the GnRHR1. |
| Databáze: |
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