| Autor: |
Arriaga, José Miguel, Cohen, Noah D., Derr, James N., Chaffin, M. Keith, Martens, Ronald J. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; July 2002, Vol. 14 Issue: 4 p347-353, 7p |
| Abstrakt: |
Species-specific primers for the polymerase chain reaction (PCR) for the detection of Rhodococcus equiwere developed. These primers were based on unique DNA fragments produced from R. equireference strains and field isolates. Following random amplification of polymorphic DNA from R. equiand R. rhodochrouswith a set of 40 arbitrary 10–base pair (bp) primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of R. equichromosomal DNA. This PCR product was limited to R. equiand was not detectable in other Rhodococcusspecies or in a panel of additional gram-positive and gram-negative bacteria. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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