| Autor: |
Cai, Hugh Y., van Dreumel, Tony, McEwen, Beverly, Hornby, Geoff, Bell-Rogers, Patricia, McRaild, Pat, Josephson, Gaylan, Maxie, Grant |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; January 2007, Vol. 19 Issue: 1 p91-95, 5p |
| Abstrakt: |
A PCR assay was validated for the detection of Mycoplasma hyopneumoniaein porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae.In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniaeinfection by M. hyopneumoniaePCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniaeinfection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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