| Autor: |
Marques, L. M., Buzinhani, M., Yamaguti, M., Oliveira, R. C., Ferreira, J. B., Mettifogo, E., Timenetsky, J. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; January 2007, Vol. 19 Issue: 1 p103-106, 4p |
| Abstrakt: |
A polymerase chain reaction (PCR) assay was developed for the detection of Mycoplasma disparin nasal mucus samples collected from calves. The target DNA sequence was the 16S rRNA gene, and the fragment was selected within a region of high polymorphism. The specificity and detection limit of the method were determined. This method was then used for the detection of M. disparin nasal swabs collected from 301 calves, including 155 clinical samples from animals showing signs of respiratory disease and 146 samples from healthy animals. PCR with generic primers was applied to the detection of Mollicutes, followed by the detection of M. dispar. Mollicuteswere detected in 52.05% of clinical samples from healthy animals and in 90.96% of samples from sick animals. Mycoplasma disparwas detected in 6.16% of healthy animals and in 34.84% of sick animals. The PCR assay was useful in verifying the presence of M. disparin calves and may be a useful tool in monitoring this mycoplasma in cattle herds. |
| Databáze: |
Supplemental Index |
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