| Autor: |
Spycher, Andrea, Geigy, Caroline, Howard, Judith, Posthaus, Horst, Gendron, Karine, Gottstein, Bruno, Debache, Karim, Herrmann, Daland C., Schares, Gereon, Frey, Caroline F. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; January 2011, Vol. 23 Issue: 1 p104-108, 5p |
| Abstrakt: |
A 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. Serologic testing for Feline immunodeficiency virusand Feline leukemia viruswere negative. Fine-needle aspirates of mesenteric lymph nodes revealed the presence of banana-shaped apicomplexan parasites. The cat died after 4 days of hospitalization. Postmortem polymerase chain reaction (PCR) analysis confirmed the presence of Toxoplasma gondiiin all examined organs. Parasites were ex vivo isolated in outbred mice and subsequently transferred into cell culture. Genotyping, using genetic markers for SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico for PCR–restriction fragment length polymorphism, revealed infection with type II T. gondiidisplaying type II alleles at all loci except Apico, which exhibited a type I allele. This is the most frequently identified genotype among cats acting as definitive hosts in central Europe, but to the authors' knowledge, it has never been associated with systemic toxoplasmosis in an adult, immunocompetent cat. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
