| Autor: |
Sornlake, Warasirin, Rattanaphanjak, Phatcharamon, Champreda, Verawat, Eurwilaichitr, Lily, Kittisenachai, Suthathip, Roytrakul, Sittiruk, Fujii, Tatsuya, Inoue, Hiroyuki |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; July 2017, Vol. 81 Issue: 7 p1289-1299, 11p |
| Abstrakt: |
Schizophyllum communeis a basidiomycete equipped with an efficient cellulolytic enzyme system capable of growth on decaying woods. In this study, production of lignocellulose-degrading enzymes from S. communemutant G-135 (SC-Cel) on various cellulosic substrates was examined. The highest cellulase activities including CMCase, FPase, and β-glucosidase were obtained on Avicel-PH101 while a wider range of enzymes attacking non-cellulosic polysaccharides and lignin were found when grown on alkaline-pretreated biomass. Proteomic analysis of SC-Cel also revealed a complex enzyme system comprising seven glycosyl hydrolase families with an accessory carbohydrate esterase, polysaccharide lyase, and auxiliary redox enzymes. SC-Cel obtained on Avicel-PH101 effectively hydrolyzed all agricultural residues with the maximum glucan conversion of 98.0% using corn cobs with an enzyme dosage of 5 FPU/g-biomass. The work showed potential of SC-Cel on hydrolysis of various herbaceous biomass with enhanced efficiency by addition external β-xylosidase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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