| Autor: |
Ness, SallyAnne L., Schares, Gereon, Peters-Kennedy, Jeanine, Mittel, Linda D., Dubey, Jitender P., Bowman, Dwight D., Mohammed, Hussni O., Divers, Thomas J. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; November 2014, Vol. 26 Issue: 6 p778-782, 5p |
| Abstrakt: |
Besnoitiosis is an emerging infectious disease of donkeys (Equus asinus) in the United States for which there are currently no serologic methods of diagnosis. A study was performed to evaluate physical examination findings and 3 serologic assays for the detection of Besnoitia bennettiinfection in donkeys. A prospective study of 416 donkeys from 6 privately owned herds across 5 U.S. states (New York, Pennsylvania, Vermont, Oregon, and Washington) was performed. Donkeys were examined for clinical lesions suggestive of besnoitiosis and evaluated for antibodies against B. bennettiusing a fluorescent antibody test (FAT) and 2 immunoblot assays specific for bradyzoite and tachyzoite antigens, respectively. Donkeys were confirmed to be infected with B. bennettiby histology (cases; n= 32) and were compared to those with no clinical signs of besnoitiosis (controls; n= 384). Identifying clinical lesions in 2 or more locations correctly identified infected donkeys 83% of the time. Donkeys with besnoitiosis had significantly higher FAT titers (P< 0.001) and numbers of bradyzoite (P< 0.001) and tachyzoite (P< 0.001) immunoblot bands than control donkeys. The sensitivity and specificity of the serologic assays for detecting besnoitiosis was 88% and 96% for FAT, 81% and 91% for bradyzoite immunoblot, and 91% and 92% for tachyzoite immunoblot, respectively. Fluorescent antibody and immunoblot assays are effective at identifying donkeys with besnoitiosis and provide a more efficient and less invasive diagnostic alternative to histology. |
| Databáze: |
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