| Autor: |
Yanthan, J., Kehie, Mechuselie, Kumaria, Suman, Tandon, Pramod |
| Zdroj: |
3 Biotech; June 2017, Vol. 7 Issue: 2 p1-5, 5p |
| Abstrakt: |
An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmanniiVahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard medium (MS with 50 mg/l citric acid and 10 mg/l ascorbic acid) supplemented with 4 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthalene acetic acid (NAA) followed by 26.8 ± 1.4 shoots in one-fourth strength SM fortified with 4 mg/l kinetin (KN) and 4 mg/l NAA. The well-developed plantlets with shoots and roots were potted in small plastic glasses filled with a mixture of sand and farmyard manure (3:1); these plantlets when transferred to a glasshouse for hardening and acclimatization showed 90% survival. |
| Databáze: |
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