Molecular identification and phylogenetic analyses of multiple nucleopolyhedrovirus isolated from Lymantria obfuscata(Lepidoptera: Lymantriidae) in India

Autor: Gani, Mudasir, Gupta, Rakesh, Zargar, Sajad, Kour, Gurmeet, Monobrullah, Md, Kandasamy, Thamilarasi, Mohanasundaram, A.
Zdroj: Applied Entomology and Zoology; August 2017, Vol. 52 Issue: 3 p389-399, 11p
Abstrakt: The Lymantria obfuscataWalker (Lyob) multiple (M) nucleopolyhedrovirus (NPV) (LyobMNPV) has been isolated and successfully applied for the management of the Indian gypsy moth, L. obfuscatain Jammu and Kashmir (J&K), India. The present work aimed to investigate the variability of LyobMNPV isolates from six localities of J&K through molecular [amplification of the polyhedrin (polh), late expression factor-8 (lef-8) and late expression factor-9 (lef-9) genes] and biological (bioassays) characterization. To identify the position of LyobMNPV in the phylogenetic tree of baculoviruses, partial sequences of the polh, lef-8and lef-9genes were determined by using the DNA sequences within their coding regions by optimizing the polymerase chain reaction with degenerate primers. The sequence alignment revealed that LyobMNPV isolates exhibited seven, five and eleven single nucleotide polymorphic sites in the case of polh, lef-8and lef-9, respectively. The phylogenetic analyses supported placing LyobMNPV with the Lymantria disparL. MNPV (LdMNPV) isolates from different countries, and showed that it was more closely related to LdMNPV than to Lymantria xylinaSwinhoe NPV and Lymantria monachaL. NPV. The contaminated diet plug bioassays using 2nd instar larvae indicated that the median lethal dose (LD50) and median survival time (ST50) of different isolates of LyobMNPV against L. obfuscatawere lower than those of LdMNPV against L. dispar. LyobMNPV was more closely related to LdMNPV but its LD50and ST50were lower than those of LdMNPV. The study provides novel information on the position of LyobMNPV in the phylogenetic tree of baculoviruses and about biological and genetic variation of Lymantriaspecies’ NPV isolates from different parts of the world.
Databáze: Supplemental Index
Externí odkaz: