| Autor: |
Bhatwadekar, Ashay D., Beli, Eleni, Diao, Yanpeng, Chen, Jonathan, Luo, Qianyi, Alex, Alpha, Caballero, Sergio, Dominguez, James M., Salazar, Tatiana E., Busik, Julia V., Segal, Mark S., Grant, Maria B. |
| Zdroj: |
American Journal of Pathology; June 2017, Vol. 187 Issue: 6 p1426-1435, 10p |
| Abstrakt: |
The brain and muscle aryl hydrocarbon receptor nuclear translocator–like protein (BMAL)-1 constitutes a major transcriptional regulator of the circadian clock. Here, we explored the impact of conditional deletion of Bmal1in endothelium and hematopoietic cells in murine models of microvascular and macrovascular injury. We used two models of Bmal1fx/fx;Tek-Cremice, a retinal ischemia/reperfusion model and a neointimal hyperplasia model of the femoral artery. Eyes were enumerated for acellular capillaries and were stained for oxidative damage markers using nitrotyrosine immunohistochemistry. LSK (lineage-negative, stem cell antigen-1–positive, c-Kit–positive) cells were quantified and proliferation assessed. Hematopoiesis is influenced by innervation to the bone marrow, which we assessed using IHC analysis. The number of acellular capillaries increased threefold, and nitrotyrosine staining increased 1.5-fold, in the retinas of Bmal1fx/fx;Tek-Cremice. The number of LSK cells from the Bmal1fx/fx;Tek-Cremice decreased by 1.5-fold and was accompanied by a profound decrease in proliferative potential. Bmal1fx/fx;Tek-Cremice also exhibited evidence of bone marrow denervation, demonstrating a loss of neurofilament-200 staining. Injured femoral arteries showed a 20% increase in neointimal hyperplasia compared with similarly injured wild-type controls. Our study highlights the importance of the circadian clock in maintaining vascular homeostasis and demonstrates that specific deletion of BMAL1in endothelial and hematopoietic cells results in phenotypic features similar to those of diabetes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
