| Autor: |
Cary, J.W., Klich, M.A., Beltz, S.B. |
| Zdroj: |
Mycologia; March 2005, Vol. 97 Issue: 2 p425-432, 8p |
| Abstrakt: |
Most aspergilli that produce aflatoxin are members of Aspergillussection Flavi,however isolates of several Aspergillusspecies not closely related to section Flavialso have been found to produce aflatoxin. Two of the species, Aspergillus ochraceoroseusand an undescribed Aspergillusspecies SRRC 1468, are morphologically similar to members of Aspergillussection Circumdati.The other species have Emericellateleomorphs (Em. astellataand an undescribed Emericellaspecies SRRC 2520) and are morphologically distinctive in having ascospores with large flanges. All these aflatoxin-producing isolates were from tropical zones near oceans, and none of them grew on artificial media at 37 C. Aflatoxins and sterigmatocystin production were quantified by high-pressure liquid chromatography (HPLC) and confirmed by HPLC-mass spectrometry (LC-MS) detection. Phylogenetic analyses were conducted on these four species using A. parasiticusand Em. nidulans,(which produce aflatoxin and the aflatoxin precursor sterigmatocystin, respectively) for comparison. Two aflatoxin/sterigmatocystin biosynthesis genes and the beta tubulin gene were used in the analyses. Results showed that of the new aflatoxin-producers, AspergillusSRRC 1468 forms a strongly supported clade with A. ochraceoroseusas does EmericellaSRRC 2520 with Em. astellataSRRC 503 and 512. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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