| Autor: |
Nakano, Kozaburo, Sugarawa, Yoichi, Motoaki, Ohashi, Harigaya, Shoichi |
| Zdroj: |
Biochemical Pharmacology; November 1986, Vol. 35 Issue: 21 p3745-3752, 8p |
| Abstrakt: |
Metabolism of pantothenic acid (PaA) in beagle dogs investigated. The dogs excreted 12.3% of the dose in the urine within 24 hr after a single oral adminstration of [3H]PaA (3 mg/kg). High performance liquid chromotoghraphic analysis of the urine showed the presence of unchanged vitamin and a major metabolite, which accounted for 60.2 and 39.8% of the urinary radioactivity resprctively. Although the metabolite was hydrolyzed by treatment with β-glucuronidase or acid phosphatase, it was found that this hydrolysis resulted from the actions of β-glucusidase contained as a contaminant in these enzyme preparations. β-Glucusidase completely hydrolyzed the metabolite to generate PaA and glucose. The metabolite was isolated and subjected to GC/MS and NMR analyses. It was identical to synthetic PaA β-glucoside, 4′-O-(β-d-glucopanthothenic acid. It was shown by the use of dog liver microsomes that PaA underwent β-glucosidation in the presence of uridine diphosphate glucose (UDPG). It is proposed that β-glucosidation by UDP-glucosyltransferase is a novel metabolic pathway of PaA in the dog. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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