Ultrastructural localization of cellulase in Trichoderma reesei using immunocytochemistry and enzyme cytochemistry.

Autor: Chapman, C M, Loewenberg, J R, Schaller, M J, Piechura, J E
Zdroj: Journal of Histochemistry and Cytochemistry; December 1983, Vol. 31 Issue: 12 p1363-1366, 4p
Abstrakt: Two components of the cellulase complex (E.C. 3.2.1.4) of the fungus Trichoderma reesei were localized at the ultrastructural level. Immunocytochemistry and enzyme cytochemistry demonstrated that cellobiohydrolase and beta-1,4 glucanase were localized within cisternae of endoplasmic reticulum and within membrane complexes of cellulose-grown hyphae. Both enzymes were also present in the culture medium. Glucose-grown control hyphae lacked enzyme-specific staining, and no enzyme activity was detected in the growth medium.
Databáze: Supplemental Index