Autor: |
Chapman, C M, Loewenberg, J R, Schaller, M J, Piechura, J E |
Zdroj: |
Journal of Histochemistry and Cytochemistry; December 1983, Vol. 31 Issue: 12 p1363-1366, 4p |
Abstrakt: |
Two components of the cellulase complex (E.C. 3.2.1.4) of the fungus Trichoderma reesei were localized at the ultrastructural level. Immunocytochemistry and enzyme cytochemistry demonstrated that cellobiohydrolase and beta-1,4 glucanase were localized within cisternae of endoplasmic reticulum and within membrane complexes of cellulose-grown hyphae. Both enzymes were also present in the culture medium. Glucose-grown control hyphae lacked enzyme-specific staining, and no enzyme activity was detected in the growth medium. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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