Two-color immunofluorescence using a fluorescence-activated cell sorter.

Autor: Loken, M R, Parks, D R, Herzenberg, L A
Zdroj: Journal of Histochemistry and Cytochemistry; July 1977, Vol. 25 Issue: 7 p899-907, 9p
Abstrakt: A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.
Databáze: Supplemental Index