Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film technique.

Autor: Waibel, R, Ginsberg, L C, Ficsor, G
Zdroj: Journal of Histochemistry and Cytochemistry; January 1984, Vol. 32 Issue: 1 p63-66, 4p
Abstrakt: A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.
Databáze: Supplemental Index