| Autor: |
Dolanski, E A, Bieber, L L, Olgaard, M K, Helmrath, T A |
| Zdroj: |
Pediatric Research; April 1978, Vol. 12 Issue: 1, Number 1 Supplement 4 p504-504, 1p |
| Abstrakt: |
The aim of the project was to investigate the ability of neonatal liver to autoregulate glucose under various glucose concentrations. Using a recirculating isolated liver perfusion technique, livers from 3-5 day old fasted piglets were perfused for 3 hours with either 0 or 300mg/dl glucose alone or in association with 10mM lactate or alanine. The perfusion system was continuously monitored for temperature, humidity, pH, pCO2, PO2and adjusted as required to mimic physiologic parameters. Serial samples were assayed for glucose, lactate, alanine and glucose (6-3H). Net production of glucose was significantly higher with Omg glucose than with 300mg/dl in the perfusate (p < 0.05). Glucose utilization by the liver did not vary with the treatments or substrates used. Lactate concentration was monitored and no difference was found between the two treatments. In both instances lactate rose from 0.45mM at the beginning, quickly plateaued at 1-1.3mM in 15-30 minutes, and remained at that level for the 3 hrs. of perfusion. When labeled lactate or alanine was presented to the liver, it was converted to glucose without a significant increase of net glucose production. Liver glycogen before and after perfusion were extremely low and did not change. These data suggest that the neonatal piglet liver has the ability to autoregulate glucose and that this mechanism can be inhibited by exogenous glucose. (Sup. by NIH Grant HD05821-06). |
| Databáze: |
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