| Autor: |
Bührer, Christoph, Stibenz, Dietger, Graulich, Johannes, Gernhold, Ulrike, Butcher, Eugene C, Dudenhausen, Joachim W, Obladen, Michael |
| Zdroj: |
Pediatric Research; September 1995, Vol. 38 Issue: 3 p336-341, 6p |
| Abstrakt: |
ABSTRACT: L-Selectin (CD62L) is a leukocyte surface membrane glycoprotein involved in extravasation and homotypic aggregation which is rapidly cleaved off after cellular activation. From culture supernatants and body fluids, soluble L-selectin (sCD62L) has been recovered with its functional activity retained. We devised a sensitive enzyme-linked immunoassay for quantitation of sCD62L which was used to measure sCD62L in umbilical cord plasma of 255 human newborns with a gestational age (GA) of 23–43 wk (median 38 wk). sCD62L levels ranged from 1.14–13.8 pmol/mL (median 7.2 pmol/mL) and showed strong correlations with GA (r = 0.71, p < 0.001), birth weight (r = 0.66, p < 0.001), and absolute neutrophil cell counts (ANC) (r = 0.62, p < 0.001) obtained from a peripheral vein within the first 6 h of life (n—153), whereas there was a weak inverse correlation with absolute normoblast counts (r = —0.27, p < 0.001). In multiple regression analysis, only GA and ANC retained a significant association with sCD62L levels (p < 0.001). Decreased sCD62L levels were found to be associated with multiple gestation (4.8 ± 2.4 pmol/mL versus 7.7 ± 2.3 pmol/mL, p < 0.05) also when considering GA and ANC as covariates. In contrast, increased sCD62L levels in infants born from meconium-stained amniotic fluid, and decreased levels in newborns with acute bacterial infection could be fully attributed to differences in GA and ANC. Umbilical cord blood sCD62L levels of healthy, term, vaginally born singletons (n = 38) were significantly lower (8.5 ± 2.2 versus 11.8 ± 1.9, p < 0.0001) than cubital vein levels of healthy adults (n = 20). We conclude that sCD62L levels display a strong increase during fetal maturation. |
| Databáze: |
Supplemental Index |
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