| Abstrakt: |
Drugs commonly used in the neonate have been implicated in the etiology of neonatal NEC. We assessed the effects of F, I and A in ischemia and reperfusion of the rabbit gut. Seven rabbits underwent laparotomy under anesthesia. In each, 8 intestinal loops (4 pairs), 5 cm long, were prepared. Each pair of loops was injected, respectively, with F (10 mg), I (0.5 mg), A (25 mg) and saline (S) (1 ml). One loop of each pair was rendered ischemic for 5 mins; the other loop with the same drug served as its non- ischemic control. The animals were sacrificed 4 hours after surgery. All intestinal loops were fixed and examined histologically by a single pathologist unaware of group assignment. Histologic changes were graded.The significant (p<0.001) findings that 6/7 ischemic loops containing F were normal and all ischemic loops with I & A were necrotic, suggest that intestinal reperfusion injury may be enhanced by I & A while F is protective, at these doses. These animal model results indicate the need for further studies in the use of these drugs in babies at risk for NEC. |
