| Autor: |
Vohra, Kiran, Singh, Inderjit, Anderson, Virginia, Rosenfeld, Warren, Lerner, Robert, Jhaveri, Ramesh |
| Zdroj: |
Pediatric Research; April 1987, Vol. 21 Issue: 4 p281A-281A, 1p |
| Abstrakt: |
Free oxygen radicals(superoxide anion, O2-) cause tissue damage in reperfusion injury of the intestine. The protective effect of a specific scavenger of O2-SOD, on weanling rabbit ileum during ischemia and reperfusion was evaluated. Twenty-three anesthetized weanling rabbits underwent laparotomy. The ileum was divided into 4 loops, each 8-10cm in length. Ischemia was induced in 2 loops by clamping the artery to the loop for 5mins; undamped loops served as controls. Eleven rabbits were pretreated with parenteral human SOD(5-10mg/kg, SC) at 0 and 16hrs. and surgery begun at 20hrs. Twelve received intraluminal SOD(10mg/kg) in 2 loops, and no SOD in 2 control loops. Animals were sacrificed 4 hrs. post surgery. Loops were fixed in formalin and examined histologically for the degree of mucosal necrosis by a pediatric pathologist who was blind to the conditions of the experiment. All rabbits given SC SOD had detectable serum levels of hSOD at 20 & 24 hrs. (.9-6.Omcg/ml).Reperfused ileal loops are protected from mucosal necrosis by both parenteral and intraluminal SOD. This may be an important etiology and therapeutic modality in NEC. |
| Databáze: |
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