| Autor: |
Berkvens, Theo M, Valerio, Dinko, Weeda, Geert, Duyvesteijn, Marja G C, Dekker, Ben M M, Ormondt, Hans V, Khan, P Meera, Eb, Alex V D |
| Zdroj: |
Pediatric Research; July 1985, Vol. 19 Issue: 7 p746-746, 1p |
| Abstrakt: |
We have cloned and characterized the human gene for adenosine deaminase (ADA)(ref.1). One surprising feature of this gene is the lack of the characteristic eukaryotic promoter elements (i.e. a TATA and a CAAT box) in the region (0-135) upstream of the cap site (+1). Nevertheless, this upstream region, which is extremely GC-rich (82%), was shown to have promoter activity in a transient expression assay (ref.1). It was hypothesized that the remarkable symmetrically disposed GC-rich motifs found in this promoter region play an important role in the regulation of the ADA gene. This was deduced from their striking homology with elements found in other genes (e.g. those for DHFR and PGK), especially with those elements in the SV-40 early promoter which have been proven to bind specific transcription factors (SP1) intimately (ref.2).To investigate the ADA promoter in more detail, we linked the promoter region to the chloramphenicol acetyl transferase (CAT) gene and determined its activity in cell lines derived from various tissues and representing different stages of differentiation. By in vitro mutagenesis of the ADA promoter and by competition experiments involving cotransfection with specific SV-40 early promoter sequences we are attempting to resolve the role of the GC-rich motifs in the functioning of this remarkable promoter. Ref.1: D.Valerio et al., The EMBO J.vol.4(2) 1985, in press.2: D.Gidoni et al., Nature 312: 409-413 (1984). |
| Databáze: |
Supplemental Index |
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