| Autor: |
Ainbender, Eugene, Drown, Edwin G, Lee, Yun J, Sweet, Avron Y |
| Zdroj: |
Pediatric Research; July 1977, Vol. 11 Issue: 7 p808-811, 4p |
| Abstrakt: |
Summary: A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in scrum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity to be 23.7 ± 0.76 (SD) mg/dl and the greatest difference between any two values was 2 mg/dl. This new technique can determine the bilirubin binding capacity of a serum sample in 15 min. Highly skilled technicians are not needed and very small quantities of serum are required, which gives it a definite advantage over the Sephadex G-25 method.Speculation: Paper chromatoelectrophoresis is a simple, rapid, and inexpensive technique which can be used to measure the bilirubin binding capacity in very small volumes of serum. It appears to have sufficient accuracy and reliability to be applied as a clinical guide to the severity of unconjugated (indirect acting) hyperbilirubinemia in the newborn infant. If further experience with the method supports this report, decisions regarding phototherapy and exchange transfusion to treat infants with unconjugated hyperbilirubinemia might be made on a more rational basis.The technique could be made much more sensitive by using radioactive bilirubin. In this way, it could become a useful research tool in the study of bilirubin metabolism. |
| Databáze: |
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