| Autor: |
Katz, J, Walter, B N, Bennetts, G A, Cairo, M S, Morgan, Beverly |
| Zdroj: |
Pediatric Research; April 1985, Vol. 19 Issue: 4 p264A-264A, 1p |
| Abstrakt: |
Helper/suppressor (H-S) cell abnormalities are found in more than 70% of hemophilia persons who are receiving factor VIII concentrates. Interleukin 2 (IL2) or T cell growth factor is a lymphokine produced by the helper cell as well as other cells. We studied 12 hemophilia subjects, 7 who received factor VIII concentrates and 5 who received cryoprecipitate. All those receiving factor VIII and 2 of the 5 receiving cryoprecipitate had reversed H-S ratios. IL2 was assayed on the supernates of PHA-stimulated mononuclear cells previously isolated on a ficoll-hypaque density gradient. The target cell was a human IL2 dependent T cell line established by stimulating normal mononuclear cells with PHA for 3 days. The cells were incubated for a further 4 days in media containing 50%, 4x concentrated IL2 previously prepared from PHA stimulated supernates pooled from normals.Significant high levels of IL2 were found in hemophilia persons receiving factor VIII concentrate and cryoprecipitate. It is possible that a receptor defect accounts for the H cell failure. |
| Databáze: |
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