| Autor: |
Ambruso, D R, Hawkins, B, Johnson, D L, Fritzberg, A R, Klingensmith, W C, McCabe, E R B |
| Zdroj: |
Pediatric Research; April 1984, Vol. 18 Issue: 1, Number 1 Supplement 4 p235A-235A, 1p |
| Abstrakt: |
Adequate levels of ATP and 2,3-DPG in stored blood are important for in vivo survival and oxygen transport of red blood cells after transfusion. 31P NMR spectroscopy allows noninvasive measurement of these compounds. In this study, biochemical assays, using coupled enzyme systems, and NMR spectroscopy performed in Nicolet 150 spectrometer operating 60.74 MHzin the pulse-Fourier transform mode were used to quantitate levels of ATP and 2,3-DPG during a five-week storage period of both whole blood (WB) and packed red blood cells (RBCs). Blood was drawn into CPD-adenine and processed according to standard blood bank procedures. Results for WB are shown below expressed as μmol/gm Hgb (mean ± SEM).Both techniques yielded similar results for ATP and 2,3-DPG with the expected decrease during storage. Results were similar for PRBC. NMR spectroscopy provides a noninvasive technique for quantitating ATP and 2,3-DPG which could be applied to evaluation of disease states and quality control of stored blood. |
| Databáze: |
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