| Autor: |
Guerina, Nicholas G, Kessler, T Woodrow, Langermann, Sol, Guerina, Victoria J, Clegg, Herbert W, Goldmann, Donald A |
| Zdroj: |
Pediatric Research; April 1984, Vol. 18 Issue: 1, Number 1 Supplement 4 p276A-276A, 1p |
| Abstrakt: |
We have purified and characterized MS pili from E. coli (EC) Kl and Klebsiella pneumoniae (KP). These pili were found to be chemically and serologically similar. In vitro adherence of both ECKl strain C94 and KP strain Fader to oropharyngeal cells was inhibited by purified MS pili from either strain. When pregnant rats were immunized with purified MS pili from either strain C94 or strain Fader, high levels of colostral and milk anti-pilus antibody were produced as determined by ELISA. The average milk titers with C94 pilus immunization were 3,000 for IgG and 2,000 for IgA; with Fader MS pili, titers were 2,500 for IgG and 2,000 for IgA. Pre-immune titers were undetectable. Infection with strain C94 was prevented in neonatal rats suckled by dams immunized with Fader as well as homologous MS pili:The highly significant heterologous protection (p<0.001), coupled with recent serological studies, suggest that life-threatening, ECK1 infection may be prevented by a vaccine composed of MS pili from a small selection of clinical isolates. KP may colonize and invade in a manner similar to ECK1, so vaccination with the same selection of MS pili may also prevent KP sepsis. |
| Databáze: |
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