| Abstrakt: |
To study the regulation of immunoglobulin (Ig) production in human lymphocytes and to link the genes for heavy and light chain synthesis to specific human chromosomes, we have developed methods for hybridization of the cells of established lymphocyte lines. Our first fusion involved an interspecific human lymphocyte (HGPRT-, kappa chain producing) × hamster fibroblast (TK-) cross; the second, an intraspecific human lymphocyte × lymphocyte cross, with both lines producers of gamma, mu, and kappa chains. In both instances, the lymphocytes were pre-treated with 0.01% trypsin prior to addition of Sendai virus. In the interspecific cross, kappa chain production was lost; in the intraspecific cross, in which the chromosomes of both parental cells were initially retained, gamma, mu, and kappa chain production were also retained, as determined by immunofluorescence. The lines resulting from the lymphocyte × lymphocyte hybridization were, however, unstable, and reverted to diploidy by 8 weeks post-fusion. We are currently attempting to establish and maintain a chromosomally stable intraspecific hybrid obtained from fusion of an Ig+and an Ig−cell line. |
