| Autor: |
McCoy, E E, Strynadka, K, Pabst, H F, Crawford, J |
| Zdroj: |
Pediatric Research; April 1982, Vol. 16 Issue: 4 p314-317, 4p |
| Abstrakt: |
Summary: Increased polyamine content is associated with increased rates of cell growth. Several Down's syndrome (D.S.) tissues have been shown to have decreased growth rates. Studies were undertaken to determine if the polyamine content of stimulated D.S. lymphocytes was similar to that of stimulated normal cells. Lymphocytes were isolated and cultured in the presence of Concanavalin A for 4 or 5 days. Polyamines were then extracted and quantitated. After 4 days spermidine content for normal cells was 930.9 ± 127 and for D.S. cells 489.2 ± 113.1 nmoles/109cells (P < 0.025). Spermine content of normal cells was 1152.8 ± 157.4 and for D.S. cells 533.9 ± 82.0 (P < 0.005). After 5 days in culture spermidine content of normal cells was 803.0 ± 75.9 and for D.S. cells 446.2 ± 76.5 nmoles/109cells (P < 0.005). Spermine content was 1155.7 ± 121.9 for normal cells and 555.1 ± 68.4 nmoles/109for D.S. cells. Decreased content of polyamines in D.S.-stimulated lymphocytes is most probably due to decreased rate of polyamine synthesis. Decreased content of polyamines in response to stimulation may be a factor in decreased growth rates and altered immune function seen in D.S. patients.Speculation: Polyamine content is associated with increased rates of DNA and cell replication. If decreased polyamine synthesis occurred in multiple tissues in Down's syndrome, it could result in a total decrease in cell number and small stature present in Down's syndrome patients. |
| Databáze: |
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