| Autor: |
Shajitha, P., Dhanesh, N., Ebin, P., Laly, Joseph, Aneesha, Devassy, Reshma, John, Augustine, Jomy, Linu, Mathew |
| Zdroj: |
3 Biotech; December 2016, Vol. 6 Issue: 2 p1-5, 5p |
| Abstrakt: |
Only a few Impatiensspp. from South India (one of the five centers of diversity for Impatiensspecies) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiensspecies of South India, by placing them in the global phylogeny of Impatiensbased on a combined analysis of two chloroplast genes. Thirty species of genus Impatienswere collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB–rbcLand trnL-Fintergenic spacer-specific forward and reverse primers. Thirteen sequences of Impatiensspecies from three centers of diversity were obtained from GenBank for reconstructing the evolutionary relationships within the genus Impatiens. Bayesian inference analysis was carried out in MrBayes v.3.2.2. This analysis supported Southeast Asia as the ancestral place of origin of extant Impatiensspecies. Molecular phylogeny of South Indian Impatiensspp. based on combined chloroplast sequences showed the same association as that of morphological taxonomy. Sections Scapigerae, Tomentosae, Sub-Umbellatae,and Racemosaeshowed Southeast Asian relationship, while sections Annuaeand Microsepalaeshowed African affinity. |
| Databáze: |
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