| Autor: |
Hu, Fang, Rishishwar, Lavanya, Sivadas, Ambily, Mitchell, Gabriel J., Jordan, I. King, Murphy, Timothy F., Gilsdorf, Janet R., Mayer, Leonard W., Wang, Xin |
| Zdroj: |
Journal of Clinical Microbiology; October 2016, Vol. 54 Issue: 12 p3010-3017, 8p |
| Abstrakt: |
ABSTRACTHaemophilus haemolyticushas been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable Haemophilus influenzae(NT H. influenzae). NT H. influenzaeand H. haemolyticusare often misidentified because none of the existing tests targeting the known phenotypes of H. haemolyticusare able to specifically identify H. haemolyticus. Through comparative genomic analysis of H. haemolyticusand NT H. influenzae, we identified genes unique to H. haemolyticusthat can be used as targets for the identification of H. haemolyticus. A real-time PCR targeting purT(encoding phosphoribosylglycinamide formyltransferase 2 in the purine synthesis pathway) was developed and evaluated. The lower limit of detection was 40 genomes/PCR; the sensitivity and specificity in detecting H. haemolyticuswere 98.9% and 97%, respectively. To improve the discrimination of H. haemolyticusand NT H. influenzae, a testing scheme combining two targets (H. haemolyticuspurTand H. influenzaehpd, encoding protein D lipoprotein) was also evaluated and showed 96.7% sensitivity and 98.2% specificity for the identification of H. haemolyticusand 92.8% sensitivity and 100% specificity for the identification of H. influenzae, respectively. The dual-target testing scheme can be used for the diagnosis and surveillance of infection and disease caused by H. haemolyticusand NT H. influenzae. |
| Databáze: |
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