| Autor: |
Neumann, Yvonne, Bruns, Svenja A., Rohde, Manfred, Prajsnar, Tomasz K., Foster, Simon. J., Schmitz, Ingo |
| Zdroj: |
Autophagy; November 2016, Vol. 12 Issue: 11 p2069-2084, 16p |
| Abstrakt: |
ABSTRACTAutophagy, a catabolic pathway of lysosomal degradation, acts not only as an efficient recycle and survival mechanism during cellular stress, but also as an anti-infective machinery. The human pathogen Staphylococcus aureus(S. aureus) was originally considered solely as an extracellular bacterium, but is now recognized additionally to invade host cells, which might be crucial for persistence. However, the intracellular fate of S. aureusis incompletely understood. Here, we show for the first time induction of selective autophagy by S. aureusinfection, its escape from autophagosomes and proliferation in the cytoplasm using live cell imaging. After invasion, S. aureusbecomes ubiquitinated and recognized by receptor proteins such as SQSTM1/p62 leading to phagophore recruitment. Yet, S. aureusevades phagophores and prevents further degradation by a MAPK14/p38α MAP kinase-mediated blockade of autophagy. Our study demonstrates a novel bacterial strategy to block autophagy and secure survival inside the host cell. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
