| Autor: |
Reddy, Madam, Senthilraja, Chinnaiah, Adhithya, Rangasamy, Satya, Vijayalakshmi, Kokiladevi, Easwaran, Sudhakar, Durailagaraja, Rabindran, Ramalingam, Velazhahan, Rethinasamy |
| Zdroj: |
Journal of Plant Diseases and Protection; October 2016, Vol. 123 Issue: 5 p205-214, 10p |
| Abstrakt: |
The feasibility of controlling peanut stem necrosis disease caused by Tobacco streak virus(TSV) in groundnut (Arachis hypogaeaL.) was explored by expressing double-stranded RNA of the replicase (Rep) gene of TSV in groundnut through genetic engineering. A hairpin (hp) RNAi construct containing 535-bp sense and antisense TSV-Repsequences flanking a 742-bp spacer sequence (Pdk intron) under the control of the constitutive Cauliflower mosaic virus35S promoter was made in the binary vector pART27. This chimeric gene construct was then mobilized into Agrobacterium tumefaciensstrain LBA4404 via triparental mating using pRK2013 as a helper. Cotyledon explants of groundnut cultivar TMV-7 were transformed with A. tumefaciensharboring the hpRNA cassette. The presence of the transgene in the transgenic plants was confirmed up to T3generation by PCR amplification of the 535-bp fragment of TSV-Repgene. The bioassay results indicated that necrotic lesions were observed on the leaves of the wild-type plants 7–9 days after inoculation with TSV and stem necrosis appeared 16–20 days after inoculation, whereas the transgenic plants did not develop symptoms until harvest. ELISA results indicated that the wild-type plants inoculated with TSV recorded the highest virus concentration as compared to the transgenic lines. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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