| Autor: |
Udayasuriyan, Varatharajalu, Nakamura, Akira, Masaki, Haruhiko, Uozumi, Takeshi |
| Zdroj: |
Journal of Fermentation and Bioengineering; January 1996, Vol. 82 Issue: 3 p306-308, 3p |
| Abstrakt: |
SDS-PAGE analysis of the insoluble fraction of a recombinant Escherichia colicell extract revealed that the DNA fragment containing the coding region of the cryIA(a)gene (3528 bp) with 80-bp upstream and 153-bp downstream flanking regions, placed under the control of the tacpromoter in pTTQ19, produces a considerable amount of CryIA(a) proteins in E. colicells grown at 37°C without IPTG induction. Under similar conditions, the presence of an additional upstream region (ca. 400 bp) of the cryIA(a)gene was found to increase the production of CryIA(a) proteins about 2-fold in E. coli. Overexpression of the cryIA(a)gene in E. coliwithout the addition of IPTG offers the advantage of reduced production costs. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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