Bioconversion of AHX to AOH by resting cells of Burkholderia contaminansCH-1

Autor: Choi, Jae-Hoon, Kikuchi, Ayaka, Pumkaeo, Panyapon, Hirai, Hirofumi, Tokuyama, Shinji, Kawagishi, Hirokazu
Zdroj: Bioscience, Biotechnology, and Biochemistry; October 2016, Vol. 80 Issue: 10 p2045-2050, 6p
Abstrakt: Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. We previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH). In this study, a bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX. The strain converted AHX to AOH and identified as Burkholderia contaminansbased on the gene sequence of its 16S rDNA. The quantitative production of AOH by resting cells of the strain was achieved. Among seven Burkholderiaspecies, two bacteria and two yeasts tested, B. contaminansCH-1 showed the highest rate of conversion of AHX to AOH. By batch system, up to 10.6 mmol AHX was converted to AOH using the resting cells. The yield of this process reached at 91%.
Databáze: Supplemental Index