In contrast to dogs, red foxes (Vulpes vulpes) did not shed Neospora caninumupon feeding of intermediate host tissues

Autor: Schares, Gereon, Heydorn, Alfred, Cüppers, Arnd, Mehlhorn, Heinz, Geue, Lutz, Peters, Martin, Conraths, Franz
Zdroj: Parasitology Research; January 2002, Vol. 88 Issue: 1 p44-52, 9p
Abstrakt: To clarify whether red foxes (Vulpes vulpes) can be final hosts of Neospora caninum, foxes and dogs were fed in parallel on tissues of a sheep and a goat experimentally infected with N. caninum. The faeces of at least two of five dogs contained N. caninumoocysts, as determined by bioassay. In the faeces of all six foxes fed in parallel, oocysts were detected that were larger in size (length 12.6±0.5 µm, width 11.8±0.4 µm) than the oocysts shed by the dogs. Ribosomal RNA sequences and the results of an immunoblot-based bioassay provided further evidence that these oocysts were different from N. caninum. A titration experiment performed to determine the sensitivity of a bioassay utilising gerbils showed that as few as five sporulated N. caninumoocysts could be detected by this test. This indicates that, in two feeding experiments, less than 3,700 and 200 sporulated N. caninumoocysts, respectively, could have been among the Hammondiasp.-like oocysts collected from fox faeces. These results suggest that the red fox is either an inappropriate final host for N. caninumor not at all a final host for this parasite.
Databáze: Supplemental Index