| Autor: |
Gherdaoui, Dehbiya, Bekdouche, Hafsa, Zerkout, Said, Fegas, Rachid, Righezza, Michel |
| Zdroj: |
Journal of the Iranian Chemical Society; December 2016, Vol. 13 Issue: 12 p2319-2323, 5p |
| Abstrakt: |
A high-performance liquid chromatographic method for chiral separation of ketoprofen racemate was developed. (R)- and (S)-ketoprofen enantiomers were separated on a LiChrosorb NH2column (250 mm × 4.6 mm, i.d 5 µm) at 20 °C, using 2-propanol/potassium dihydrogen phosphate buffer (pH 6.0, 0.05 M) (50:50 v/v). Containing vancomycin as the mobile phase, at a flow rate of 0.8 ml min−1and detection wavelength of UV, the detector was set at 310 nm. Under these conditions, ketoprofen enantiomers could be separated with a selectivity factor (α) of 2.172 and a resolution (Rs) of 4.78 using extremely low concentrations of the vancomycin chiral additive. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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