| Autor: |
Costa, Bruna Zucoloto da, Rodrigues, Viviane Drumond, Oliveira, Valéria Maia de, Ottoboni, Laura Maria Mariscal, Marsaioli, Anita Jocelyne |
| Zdroj: |
Brazilian Journal of Microbiology; October-December 2016, Vol. 47 Issue: 4 p846-852, 7p |
| Abstrakt: |
Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaã dos Carajás, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonasand Stenotrophomonas.The bacterial strains B. megaterium(SO5-4 and SO6-2) and Pseudomonassp. (SO5-9) efficiently oxidized three different organic sulfides to their corresponding sulfoxides. In conclusion, this study revealed that neutral copper mine drainages are a promising source of biocatalysts for ester hydrolysis and sulfide oxidation/bioremediation. Furthermore, this is a novel biotechnological overview of the heterotrophic bacteria from a copper mine drainage, and this report may support further microbiological monitoring of this type of mine environment. |
| Databáze: |
Supplemental Index |
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