| Autor: |
Foster, D.N, Proudman, J.A, Harmon, S.A, Foster, L.K |
| Zdroj: |
Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology; June 1997, Vol. 117 Issue: 2 p233-239, 7p |
| Abstrakt: |
A full-length chicken growth hormone (cGH) cDNA was placed downstream from the Autograph californicanuclear polyhedron virus, AcNPV, polyhedron gene promoter and expressed in Sf9 insect cells. Secreted recombinant cGH levels averaged 2–10 μg/ml from day 5–10 postinfection. The recombinant cGH analyzed by SDS-PAGE gels and Western blotting consisted of a doublet with Mrof 26.5 and 23.5 kDa. Analysis by 2-D electrophoresis of partially-purified recombinant cGH and purified native cGH revealed similar immunoreactive charge isoforms and Mrvariants. The recombinant hormone was biologically active in a homologous radioreceptor assay. The results show that cGH expressed in insect cells is biologically and immunologically active, and that a variety of isoforms are secreted which exhibit size and charge properties similar to those of pituitary-derived cGH. |
| Databáze: |
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