| Autor: |
Yamada, Koji, Kazama, Yusuke, Mitra, Sharbanee, Marukawa, Yuka, Arashida, Ryo, Abe, Tomoko, Ishikawa, Takahiro, Suzuki, Kengo |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; August 2016, Vol. 80 Issue: 8 p1650-1656, 7p |
| Abstrakt: |
Euglena gracilisis a common phytoplankton species, which also has motile flagellate characteristics. Recent research and development has enabled the industrial use of E. gracilisand selective breeding of this species is expected to further expand its application. However, the production of E. gracilisnuclear mutants is difficult because of the robustness of its genome. To establish an efficient mutation induction procedure for E. gracilis, we employed Fe-ion beam irradiation in the RIKEN RI beam factory. A decrease in the survival rate was observed with the increase in irradiation dose, and the upper limit used for E. gracilisselective breeding was around 50 Gy. For a practical trial of Fe-ion irradiation, we conducted a screening to isolate high-temperature-tolerant mutants. The screening yielded mutants that proliferated faster than the wild-type strain at 32 °C. Our results demonstrate the effectiveness of heavy-ion irradiation on E. gracilisselective breeding. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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