| Autor: |
Vargas, Manuel A., Bertrand, Francoise, Mulongo, Kipanga N., Squifflet, Jean P., Lamy, Monique E. |
| Zdroj: |
Clinical and Diagnostic Virology; 1996, Vol. 6 Issue: 1 p1-9, 9p |
| Abstrakt: |
Background: Specific HCMV IgE response has been reported by some authors, and was proposed as a valuable virologic marker of CMV infection. Objectives: we evaluated specific HCMV IgE in renal transplant patients with active (primary and secondary) HCMV infection with special interest to symptomatic infections. Study design: Specific IgE was tested retrospectively by ELISA and immunoblot (IB) on sera of 55 RT patients who were followed before and after transplantation with virologic markers of CMV infection. Results: Total serum IgE levels were similar in control group and in patients with primary and secondary HCMV infections. Anti-CMV specific IgE response by ELISA was more frequently found in patients with primary infection (76.9%) than in patients with secondary infection (47.1%). These specific IgE reacted on immunoblot with a 150 kDa protein in 84.6% of patients with primary infection and 94.1% with secondary infections; and reacted with rp52 (pUL44) in 76.9% of primary infection and 47.1% of secondary infection. Conclusions: Anti-CMV specific IgE tested by immunoblot and ELISA is a marker of CMV infection. It was clearly detected in cases of active infection (primary and secondary) and was present in cases with severe CMV clinical manifestations. In contrast, anti-CMV specific IgE, was consistently negative among healthy blood donors. This is the first report of CMV proteins detected by IgE immunoblot. |
| Databáze: |
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