| Autor: |
Freeman, Thomas C., Curry, Brenda J., Calam, John, Woodburn, James R. |
| Zdroj: |
Gastroenterology; November 1990, Vol. 99 Issue: 5 p1414-1420, 7p |
| Abstrakt: |
Pancreatic secretory trypsin inhibitor was examined for growth-promoting activity on five cell lines using standard cell culture techniques. One cell line, AR4-2J, derived from a rat pancreatic acinar cell carcinoma, responded with significantly increased incorporation of [3H]thymidine and colony formation. Pancreatic secretory trypsin inhibitor stimulated the incorporation of [3H]thymidine in liquid culture; the maximal increase was 61 ± 10% above control (P< 0.001) and was seen at a concentration of 10−9mol/L. Using a soft agarose clonogenic assay, pancreatic secretory trypsin inhibitor also consistently stimulated (3 assays) colony formation: the peak activity occurred at a concentration of 10−10mol/L which caused a 150 ± 55% (mean ± SE, P< 0.05) increase above control. Aprotinin had no effect on the growth of AR4-2J cells and pancreatic secretory trypsin inhibitor did not bind to the epidermal growth factor receptor. AR4-2J cells were shown to produce pancreatic secretory trypsin inhibitor. The study raises the possibility that pancreatic secretory trypsin inhibitor provides autocrine stimulation of tumor cell growth. |
| Databáze: |
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