| Autor: |
Higaki, Ikko, Matsui-Yuasa, Isao, Terakura, Masanobu, Kinoshita, Hiroaki, Otani, Shuzo |
| Zdroj: |
Gastroenterology; April 1994, Vol. 106 Issue: 4 p1024-1031, 8p |
| Abstrakt: |
Background/Aims:Hepatocyte growth factor is a potent mitogen for mature hepatocytes and seems to act as a trigger for liver regeneration. Hepatocyte growth factor was first purified from human and rabbit plasma and rat platelets. Additionally, putrescine, spermidine, and spermine are widely distributed in many different cells; intracellular concentrations of these polyamines are closely related to cell proliferation. The present study examined whether polyamine metabolism is involved in hepatocyte growth factor-induced DNA synthesis in primary cultured rat hepatocytes. Methods:Hepatocytes were isolated from rats by the collagenase perfusion method. Ornithine decarboxylase and S-aden-osylmethionine decarboxylase activities were measured as the release of 14CO2from l-[1-14C]ornithine and S-adenosyl-l-[carboxyl-14C]methionine, respectively. Results:α-Difluoromethylornithine inhibited hepatocyte growth factor-induced DNA synthesis by only 21%. On the other hand, methylglyoxal bis(guanylhydra-zone) completely inhibited hepatocyte growth factor-induced DNA synthesis to nontreated control level. The inhibitory effect of methylglyoxal bis(guanylhydrazone) on hepatocyte growth factor-induced DNA synthesis was reversed by exogenously added spermidine or spermine. Conclusions:Spermidine or spermine is essential for hepatocyte growth factor-induced DNA synthesis in primary cultured rat hepatocytes. |
| Databáze: |
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