| Autor: |
Karnes, William E., Walsh, John H., Vincent Wu, S., Kim, Richard S., Martin, Martin G., Wong, Helen C., Mendelsohn, John, Park, Jae-Gahb, Cuttitta, Frank |
| Zdroj: |
Gastroenterology; February 1992, Vol. 102 Issue: 2 p474-485, 12p |
| Abstrakt: |
Four human colon adenocarcinoma cell lines, SNU-C1, SNU-C4, SNU-C5, and NCI-H716, that are capable of proliferating autonomously in serum-free medium containing no added peptide growth factors were identified. All four cell lines show epidermal growth factor (EGF) receptors (EGFRs), express transforming growth factor α (TGF-α) messenger RNA, and release anti-TGF-α-immunoreactive molecules. The blocking anti-EGFR monoclonal antibody (mAb) 225 blocks autonomous proliferation of SNU-C1 and SNU-C4 cells. In both of these cell lines, the inhibitory effect of mAb 225 is reversible by the addition of EGF, TGF-α, or conditioned medium from any of the four cell lines. In contrast, autonomous proliferation of SNU-C5 and NCI-H716 cells is not inhibited by mAb 225 and is not affected by exogenous EGF, TGF-α, or conditioned medium. Together, these data confirm the previous finding that anti-EGFR antibodies can inhibit the proliferation of some carcinoma cell lines that coexpress TGF-α and EGFR. However, here it is shown that the mechanisms of autonomous proliferation of colon carcinoma cell lines are heterogeneous and not always sensitive to antibody disruption of TGF-α/ EGFR autocrine interactions. |
| Databáze: |
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