Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody**This work was supported in part by the Contraceptive Research and Development (CONRAD) Program, Eastern Virginia Medical School, under a Cooperative Agreement (DPE-2044-A-00-6063-00) with the United States Agency for International Development (A.I.D.). The views expressed by the author(s) do not necessarily reflect the views of A.I.D.

Autor:	Coddington, Charles C., Fulgham, David L., Alexander, Nancy J., Johnson, Deborah, Herr, John C., Hodgen, Gary D.
Zdroj:	Fertility and Sterility; September 1990, Vol. 54 Issue: 3 p504-508, 5p
Abstrakt:	A monoclonal antibody, T-6, useful for detecting acrosome-reacted sperm based on an immunofluorescent assay, was employed to evaluate acrosomal status of human sperm that were tightly bound to hemisected human zonae pellucidae (hemizona assay). Over 90% of the bound sperm evaluated exhibited immunofluorescent patterns indicative of acrosome reaction. This staining method for evaluating the acrosomal status of sperm bound to the zona pellucida may enable definition of a group of male infertility patients heretofore not recognized.
Databáze:	Supplemental Index
Externí odkaz:	Zobrazit plný text záznamu