| Autor: |
Leibo, S.P., Giambernardi, Troy A., Meyer, Thomas K., Bastias, M. Cristina, Rogers, B. Jane |
| Zdroj: |
Fertility and Sterility; May 1990, Vol. 53 Issue: 5 p906-912, 7p |
| Abstrakt: |
To solve the logistical problems of the sperm penetration assay (SPA) to provide just a sufficient number of hamster ova exactly when they are needed, a new method to cryopreserve the ova has been devised. The ova, suspended in a 1.5M solution of propylene glycol as a cryoprotectant in an isotonic salt solution, were frozen in 14mL plastic straws. Included in each straw was a sucrose solution, isosmotic to the propylene glycol solution, to serve as an osmotic buffer during dilution of the cryoprotectant out of the ova. This one-step method of dilution permitted the ova to be recovered and diluted out of the cryoprotectant within the straw in which they had been originally frozen. A total of 547 cryopreserved ova were thawed, 504 (92.1%) of which were morphologically normal after they had been incubated at 37°C for 3 hours. After removal of the zonae, the frozen-thawed ova were compared with fresh, control ova in SPAs of donor and patient semen that had been capacitated in TEST-yolk buffer. The percent penetration and penetration index of fresh versus cryopreserved ova did not differ significantly for either donor or patient semen. |
| Databáze: |
Supplemental Index |
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