| Autor: |
Rush, B., Parkin, J.D., Carroll, Nanette |
| Zdroj: |
Pathology; January 1971, Vol. 3 Issue: 1 p71-71, 1p |
| Abstrakt: |
Current methods for the visualization of blood cells, although ranging in power of resolution from optical to ultrastrucutral level, are limited to a two-dimensional presentation. Much important information has been gained by use of these techniques, but many points of controversy remain. This study described the surface morphology of erythrocytes, leucocytes and platelets in circulating blood and bone marrow as visualized by the scanning electron microscope. Bone marrow aspirate and sequestrenated whole blood were fixed in phosphate-buffered 0.5% glutaraldehyde (pH 7.3) at 4° for 3 nr. After washing in distilled water, thin films of the cells and fragments were deposited and dried on glass slides. The slides were trimmed to a size suitable for attachment to the aluminium specimen stub and after attachment were covered with a layer of gold and examined in the scanning electron microscope. Alternative methods of fixation were undertaken, and in some instances the films were stained with Romanowsky stains or toluidine blue after glutaraldehyde fixation or smeared on glass slides initially and stained with similar stains before glutaraldehyde fixation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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